DNA

Part:BBa_K2100058

Designed by: Julia Goupil   Group: iGEM16_MIT   (2016-10-19)


Sensor 34C

This part contains two main transcriptional units. One encoding hef1a-mKate adjacent to 4 miRNA target sites. The other encodes hef1a-BFP. mKate production signifies the amount of repression occurring due to miRNA activity. BFP can be used as transfection marker.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 763
    Illegal EcoRI site found at 1416
    Illegal EcoRI site found at 2645
    Illegal EcoRI site found at 3441
    Illegal EcoRI site found at 7154
    Illegal EcoRI site found at 7782
    Illegal EcoRI site found at 8334
    Illegal XbaI site found at 8340
    Illegal SpeI site found at 5279
    Illegal PstI site found at 839
    Illegal PstI site found at 1122
    Illegal PstI site found at 1771
    Illegal PstI site found at 2276
    Illegal PstI site found at 3978
    Illegal PstI site found at 8319
    Illegal PstI site found at 8630
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 763
    Illegal EcoRI site found at 1416
    Illegal EcoRI site found at 2645
    Illegal EcoRI site found at 3441
    Illegal EcoRI site found at 7154
    Illegal EcoRI site found at 7782
    Illegal EcoRI site found at 8334
    Illegal SpeI site found at 5279
    Illegal PstI site found at 839
    Illegal PstI site found at 1122
    Illegal PstI site found at 1771
    Illegal PstI site found at 2276
    Illegal PstI site found at 3978
    Illegal PstI site found at 8319
    Illegal PstI site found at 8630
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 763
    Illegal EcoRI site found at 1416
    Illegal EcoRI site found at 2645
    Illegal EcoRI site found at 3441
    Illegal EcoRI site found at 7154
    Illegal EcoRI site found at 7782
    Illegal EcoRI site found at 8334
    Illegal BglII site found at 186
    Illegal BglII site found at 2025
    Illegal BglII site found at 3521
    Illegal BglII site found at 7862
    Illegal BglII site found at 9594
    Illegal BamHI site found at 10248
    Illegal XhoI site found at 721
    Illegal XhoI site found at 2424
    Illegal XhoI site found at 7110
    Illegal XhoI site found at 9993
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 763
    Illegal EcoRI site found at 1416
    Illegal EcoRI site found at 2645
    Illegal EcoRI site found at 3441
    Illegal EcoRI site found at 7154
    Illegal EcoRI site found at 7782
    Illegal EcoRI site found at 8334
    Illegal XbaI site found at 8340
    Illegal SpeI site found at 5279
    Illegal PstI site found at 839
    Illegal PstI site found at 1122
    Illegal PstI site found at 1771
    Illegal PstI site found at 2276
    Illegal PstI site found at 3978
    Illegal PstI site found at 8319
    Illegal PstI site found at 8630
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 763
    Illegal EcoRI site found at 1416
    Illegal EcoRI site found at 2645
    Illegal EcoRI site found at 3441
    Illegal EcoRI site found at 7154
    Illegal EcoRI site found at 7782
    Illegal EcoRI site found at 8334
    Illegal XbaI site found at 8340
    Illegal SpeI site found at 5279
    Illegal PstI site found at 839
    Illegal PstI site found at 1122
    Illegal PstI site found at 1771
    Illegal PstI site found at 2276
    Illegal PstI site found at 3978
    Illegal PstI site found at 8319
    Illegal PstI site found at 8630
    Illegal NgoMIV site found at 924
    Illegal NgoMIV site found at 1207
    Illegal NgoMIV site found at 2159
    Illegal NgoMIV site found at 8545
    Illegal NgoMIV site found at 8826
    Illegal NgoMIV site found at 9728
    Illegal AgeI site found at 1537
    Illegal AgeI site found at 8674
    Illegal AgeI site found at 9106
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7133
    Illegal BsaI site found at 11020
    Illegal BsaI.rc site found at 98


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